Allele Specific and Locus Non-specific Suppressors in Aspergillus nidulans

Novel sexual-cycle-specific gene silencing in Aspergillus nidulans.

We report a novel sexual-cycle-specific gene-silencing system in the genetic model Aspergillus nidulans. Duplication of the mating type matA(HMG) gene in this haploid organism triggers Mat-induced silencing (MatIS) of both endogenous and transgenic matA genes, eliminates function of the encoded SRY structural ortholog, and results in formation of barren fruiting bodies. MatIS is spatiotemporall...

The mechanism of action of proline suppressors in Aspergillus nidulans.

Application of Allele Specific Markers in Determining Alleles at VRN-1 Loci in Bread Wheat Genotypes

    Wheat is the most important crop and is cultivated all over the world. Wheat has a wide range of adaptability to different climates. Vernalization genes are one of the important factors determining wheat adaptability. in wheat breeding program understanding the alleles at vernalization requirement gens are useful to introduce new cultivar for different climates. At the molecular level, the ...

Molecular characterization of the Aspergillus nidulans yA locus.

We investigated the molecular organization of the region of Aspergillus nidulans chromosome I containing yA, a gene encoding the developmentally regulated enzyme conidial laccase. DNA fragments were identified that complemented the yA2 mutation and were shown to correspond to yA by genetic mapping and gene disruption experiments. The molecular map of the region was oriented to the genetic map b...

Detection and identification of translocations by increased specific nondisjunction in Aspergillus nidulans.

A meiotic technique for visual detection of translocations has been applied to ten mitotically identified interchanges, and three new translocations were discovered using this method. Testcrosses between "standard" strains and potential translocation strains-e.g. strains with newly induced mutants or descendants from translocation crosses-are inspected for the frequency of abnormal-looking colo...